Antimicrobial effect of ethanolic, methanolic, and ethyl acetate extracts of green tea against Escherichia coli and Listeria monocytogenes

Document Type : Original Article

Authors

1 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

2 Department of Food Sciences and Technology, Zahedan University of Medical Sciences, Zahedan, Iran.

3 PhD of Feed Hygiene, Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

4 Department of clinical biochemistry, Faculity of Medicine, Zabol University of Medical Sciences, Zabol, Iran.

5 Center of Agricultural Biotechnology, University of Zabol, Zabol, Iran

Abstract

 
Background and aims: One of the most important bacterial species transmitted through the use of aquatic products is Escherichia coli. The main aim of this study was to investigate the antimicrobial effects of ethanolic, methanolic, and ethyl acetate extracts of green tea on Escherichia coli and Listeria monocytogenes.
 
Methods: The samples of Escherichia coli and Listeria monocytogenes were purchased from the company and an antibiotic resistance pattern was determined. Finally, the minimum inhibitory concentration and minimum bactericidal concentration of ethanolic, methanolic, and ethyl acetate extracts of green tea were investigated.
 
Results: The results of this study showed that the lowest inhibitory concentration is related to ethyl acetate extract of green tea against E. coli, while the highest inhibitory concentration is related to methanolic extract of green tea against Listeria monocytogenes.
 
Conclusion: It is worth mentioning that high concentrations of ethanolic, methanolic, and ethyl acetate can be used as a natural antibacterial in fish products.

Keywords

Main Subjects


1. Javadian SR. Effect of Different Degradation Methods on Chemical, Microbial, Physical Sensory Characteristics of Caspian Sea and Rainbow Trout, Doctoral dissertation of Islamic Azad University, Sci and Res Branch of Tehran. 2011; 91.
2. Henry YM, Natrajan N, Lauer WF. Detex for detection of Escherichia coli O157 in raw ground beef and raw ground poultry, J AOAC Int. 2001; 84(3): 752-60.
3. Lee GY, Jang HI, Hwang IG, Rhee MS. Prevalence and classification of pathogenic Escherichia coli isolated from fresh beef, poultry, and pork in Korea, Int J Food Microbiol. 2009; 134(3): 196-200.
4. Holko I, Bisova T, Holkova Z, Kmet V. Virulence markers of Escherichia coli strains isolated from traditional cheeses made from unpasteurised sheep milk in Slovakia, Food Control. 2006; 17(5): 393–6.
5. Sadiq E, Almasi A, Bohlouli Oskouei S. Survey the microbial total count and Listeria monocytogenes on fresh fish in Kermanshah. J Mar Sci Tech.2010; 9(3): 30-35.
6. Farrokh Eslamlo H, Hami M, Athari S, Haji Mohammadi B, Hosseini Jazani N. The evaluation of contamination rate with E.coli, Staphylococcus aureus, Listeria momocytogenesis and salmonella SP. in handmade butters in urmia city. J Urmia Nurs Midwifery Fac. 2009; 7(3): 157-165. 7. Fisheries. Iranian Fisheries Statistics . Deputy Director General of Planning and Development of Iran Fisheries Organization. 2011; 28.
8. Sattari M, Shahsouni D, Shafiei Sh Fisheries (2). Honorable publication p 2008.
9. Vosoughi GH, Mustaheir B. Freshwater fish. Tehran University Press. 2009; 317.
10. Francis G, Makkar HPS, Becker K. Effects of Quillaja saponins on growth, metabolism, egg production, and muscle cholesterol in individually reared Nile tilapia (Oreochromis niloticus). Comparative Biochemistry and Physiology. 2001; 129: 105-114.
11. Mohammad A, Bano Faruqi F, Mustafa J. Edible compounds as antitumor agents. Indian J Sci Technol. 2009; 2(5): 62-74.
12. Igbinosa O, Igbinosa E, Aiyegoro O. Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). African J of Pharmacy and Pharmacology. 2009; 3(2): 058-62.
13. Donsì F, Annunziata M, Sessa M, Ferrari G. Nanoencapsulation of essential oils to enhance their antimicrobial activity in foods. LWT,- Food Sci and Tec. 2001; 44: 1908-1914.
14. Su P, Henriksson A, Nilsson C, Mitchell H. Synergistic effect of green tea extract and probiotics on the pathogenic bacteria, Staphylococcus aureus and Streptococcus pyogenes. World J of Micro and Bio. 2008; 24: 1837-1842.
15. Zou Lq, Liu W, Liu W, Liang R, Li T, Liu C, et al. Characterization and bioavailability of tea polyphenol nanoliposome prepared by combining an ethanol injection method with dynamic high-pressure microfluidization. J of Agricultural and Food Chem. 2014; 62: 934-941.
16. Fujiki H. Two stages of cancer prevention with green tea. J Cancer Res Clin Oncol. 1999; 11(125): 589-97.
17. Almajano MP, Carbo R, Jimenez JAL, Gordon MH. Antioxidant and antimicrobial activities of tea infusions. Food Chem. 2008; 108: 55-63.
18. Yukihiko H. Green tea Tokyo Food Techno Co, Ltd. (Mitsui Nor in Co., Ltd.) Tokyo, Japan. 2001; 264.
19. Nataro JP. Atypical enteropathogenic Escherichia coli: typical pathogens? Ernerg Infect Dis, 2006; 12: 6960.
20. Zhao WH, Hu ZQ, Haray shimamura, T. inhibition by epigallo catechin gallate (EGcgl) of conjugative R plasmid transfer in Escherichia coli. J Infecet Chemother. 2001; 7: 195-7.
21. Forbes BA, Sahm DF, Weissfeld AS, Trevino EA. Methods for testing antimicrobial effectiveness. In: Bailey and Scott's Diagnostic Microbiology. (Eds E.J. Baron, L.R. Peterson and S.M. Finegold), Mosby Co: St Louis, Missouri. 1990; 171-94
22. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing (2002), 16th International supplement.; 1997
23. Khalaji N, Neyestani N .Study of the inhibitory effects of green and black tea on the growth of E. coli pathogenic bacteria in laboratory environment. Iranian J of Nutrition and Food Tec. 2006; 1(3): 33-38.
24. Nasrollahi Z, Yemagari M, Moazzeni SM . Antifungal Effects of Green Tea Leaf Poliphenols on Candida Albicans. Modares Med J Biological pathology. 2009; 12(3): 71-77.
25. Ranjbar F, Islamic G, Gharemanlou A, Taheri S, Ayatollah M, Islamic Q . Effect of Green Tea Extract on Streptococcus mutans and Enterococcus fecalis in dental plaques and comparison with chlorhexidine mouthwash and sodium hypochlorite. J of Mashhad Dental School. 2015; 4: 39.
26. Elvin-Lewis MV, Kopjas T. Anticariogenic potential of commercial teas. J Prosthet Dent. 1980; (6): 273-6.
27. Rimondia S, Percival D, Monty S, Duggal S, Philip D. The effect of cocoa polyphenols on the growth, metabolism, and biofilm formation by Streptococcus mutans and Streptococcus sanguinis. Eur J Oral Sci. 2006; 114(4): 343-8.
28. Hara Y, Ishigami T. Nippon Shokuhin Kogyo Gakkaish. 1989; 36: 996–999.
29. Nodoct B, Nori N, Gandhi, Nasrabadi H, Akhundzade Basti, A. Nanocapsulation of green tea extract using Thin Film layer and its antioxidant and antimicrobial properties . Healthy Food. 2016.
30. Amarowicz R, Pegg R, Bautista D. Antibacterial activity of green tea polyphenols against Escherichia coli K 12. Food/Nahrung. 2000; 44: 60-62.
31. Yamamoto T, Juneja LR, Kim M. Chemistry and applications of green tea, CRC press. 1997.
32. Noriyuki I, Toshiyoshi A, Yutaka T, Misa I, Akifumi N, Nobuyuki Mepur HR, et al. Epicatechins purified from green tea (Camellia sinensis) differentially suppress growth of genderdependent human cancer cell lines. Evid Based Complement Alternat Med. 2006; 3(2): 237-247.
33. Mohammadzadeh B, Rezaei M. Effect of polyphenols green tea on. Microbial and chemical change rainbow trout (Oncorhynchus mykiss) during storage in ice. JFST. 2013; 38(10): 1-9.
34. Boran H, Çiftci C, Er A, Köse O, Zeki Kurtoğlu I, Kayış S. Evaluation of Antibacterial Activity of Green Tea (Camellia sinensis L.)Seeds Against Some Fish Pathogens in Rainbow Trout (Oncorhynchus mykiss, Walbaum). Turkish J of Fisheries and Aquatic Sci. 2015; 15: 49-57.
35. P. Anita P, Shyam Sivasamy PD. Madan Kumar I. Nanda Balan, and Ethiraj S. In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. J Basic Clin Pharm. 2015; 6(1): 35–39.
36.Vasudeo Z, Sonika B. Antimicrobial Activity of Tea (Camellia sinensis). Biomed. Pharmacol. 2009; 2(1): 173- 175.
37. Farooqui A, Khan A, Borghetto I, Kazmi SU, Rubino S, Paglietti B. Synergistic Antimicrobial Activity of Camellia sinensis and Juglans regia against Multidrug-Resistant Bacteria. PLoS ONE. 2015; 10(2): e0118431. https://doi.org/10.1371/journal.pone.0118431
38. Sepehri Z, Hassanshahian M, Shahi Z, Kiani Z, Nasiri AA, Dorohi Zabol MA, Sohil Baigi G. Antibacterial effect of ethanol extract of Camellia sinensis against E.coli. Asian Pacific J of Micro Res. 2014; 2(1): 6-8.