Anti-leishmanial activity of Pelargonium roseum Essential oil on the in vitro growth of Leishmania infantum promastigotes in comparison to Glucantime

Document Type: Original Article


1 1- Faculty of Veterinary Medicine, Amol University of Special ModernTechnologies, Amol, Iran

2 Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Young Researcher and Elite Club, Babol Branch, Islamic Azad University, Babol, Iran

4 Department of Veterinary Parasitology, Islamic Azad University, Babol-Branch, Babol, Iran


Background and aims: Visceral leishmaniasis so called Kala-azar is one of the important health care problems in the world. Some herbal drugs are noticed because of toxicity and drug resistance against pentavalent antimonials as used in treatment of Kala-azar. In this study, anti-leishmanial activity of Pelargonium roseum Essential oil on the in vitro growth of Leishmania infantum promastigotes in comparison to Glucantime was studied.
Methods: Different concentrations of Pelargonium roseum essential oil on culture of Leishmania infantum promastigote were surveyed with Anti-leishmania activity assays at 24 hr and 48 hr at 570 nm wavelength. All the data were analyzed by using SPSS version and anti-leishmanial potency of the oil extract of Geranium in different concentration were compared with standard dose of Glucantime.
Results: All concentration in 24 hr showed no remarkable difference. 25, 50 and 100 µL/ml efficacy was same as Glucantime on the growth of promastigotes in 24 hr MTT. All concentrations except 1µL/ml in 48hr MTT had resemble impact on the growth of promastigotes. 5 µL/ml concentration of Pelargonium roseum essential oil was significant difference effect on the parasite (P= 0.043) in 48hr.
Conclusion: Pelargonium roseum essential oil doesn’t treat Leishmania infantum promastigote quickly. After 48 hr, 5 µL/ml concentration can be a suitable candidate in clinical trials.


Main Subjects

1.Bhattacharya SK, Dash AP. Treatment of visceral leishmaniasis: options and choice. The Lancet Infec Dis. 2016; 16(2): 142-3.

2.Sarkari B, Naraki T, Ghatee MA, Khabisi SA, Davami MH. Visceral Leishmaniasis in Southwestern Iran: A Retrospective Clinico-Hematological Analysis of 380 Consecutive Hospitalized Cases (1999–2014). PloS one. 2016; 11(3): e0150406.

3.Mohebali M, Javadian E, Yaghoobi Ershadi M, Akhavan A, Hajjaran H, Abaei M. Characterization of Leishmania infection in rodents from endemic areas of the Islamic Republic of Iran. East Mediterr Health J. 2004; 10(4-5): 591-9.

4.Sarkari B, Hatam G, Adnani S, Asgari Q. Seroprevalence of feline leishmaniasis in areas of Iran where Leishmania infantum is endemic. Ann Trop Med Parasitol. 2009; 103(3): 275-7.

5.Mohebali M. Visceral leishmaniasis in Iran: review of the epidemiological and clinical features. Iran j parasitol. 2013; 8(3): 348.

6.Fakhrie-Kashan Z, Arbabi M, Delavari M, Taghi-Zadeh M, Hooshyar H, Solaymani F. The effect of aqueous and alcoholic extracts of Pelarqonium roseum on the growth of Trichomonas vaginalis in vitro. KAUMS Journal (FEYZ). 2014; 18(4): 369-75.

7. Frézard F, Demicheli C, Ribeiro RR. Pentavalent antimonials: new perspectives for old drugs. Molecules. 2009; 14(7): 2317-36.

8. Kozan E, Akkol EK, Süntar I. Potential anthelmintic activity of Pelargonium endlicherianum Fenzl. ethnopharmacol. 2016;187:183-6.

9. Oryan A. Plant-derived compounds in treatment of leishmaniasis. Iran J Vet Res. 2015; 16(1): 1.

10. Mansour R, Haouas N, Kahla-Nakbi AB, Hammami S, Mighri Z, Mhenni F. The Effect of Vitis vinifera L. Leaves Extract on Leishmania infantum In-vitro. Iran J Pharm Res. 2013;12(3):349-55.

11. Machado M, Dinis A, Santos-Rosa M, Alves V, Salgueiro L, Cavaleiro C. Activity of Thymus capitellatus volatile extract, 1, 8-cineole and borneol against Leishmania species. Veterinary parasitol. 2014;200(1):39-49.

12. Colares AV, Almeida-Souza F, Taniwaki NN, Souza CdSF, da Costa JGM, Calabrese KdS. In vitro antileishmanial activity of essential oil of Vanillosmopsis arborea (Asteraceae) baker. J Evid Based Complementary Altern Med. 2013;2013.

13. Miller MA. Rose Geranium. Alternative Medicine. 2016(28):58.

14. Rodrigues IA, Azevedo MM, Chaves FC, Bizzo HR, Corte-Real S, Alviano DS. In vitro cytocidal effects of the essential oil from Croton cajucara (red sacaca) and its major constituent 7-hydroxycalamenene against Leishmania chagasi. BMC complementary and alternative medicine. 2013;13(1):1.