Immunosuppressive activity of terpenoids from Mesua ferrea, Ficus benghalensis and Butea frondosa on human whole blood

Document Type: Original Article

Authors

VSBT, Baramati

Abstract

Background and aims: Terpenoids are one of the largest groups of natural compounds and is generally used for the treatment of human diseases e.g. taxol (anticancer drug) and artimesinin (antimalarial drug). Thus, these agents (terpenoids) derived from medicinal plant products that are effective against intracellular or extracellular infections and these are urgently required. Therefore, this research was directed to assess its immunosuppressive activity of crude terpenoids on hepatitis B vaccine containing surface antigen (HBsAg) on human whole blood.

Methods: In this study, crude terpenoids (6.25-25 mg/ml; 50 µl) extricate from the leaves of these medicinal plants and evaluated its immunosuppressive activity on HBsAg 
(20 µg/ml) using human whole blood in order to determine the analysis of blood 
counts (flow cytometry), cytotoxicity assay (MTT (3-[4,5-dimethylthiazol–2-yl]-2, 
5-diphenyltetrazolium bromide) and nitric oxide (NO) production from cell culture supernatant.

Results: The results showed that these terpenoids showed drastic decrease in monocytes and granulocytes count but increased in lymphocytes count and inhibited NO production at higher doses (25 mg/ml; 50 µl).

Conclusion: These terpenoids exhibited immunosuppressive activity and could be a promising source of medicinally important natural compound.

Keywords

Main Subjects


1. Gupta A, Khamkar PR, Chaphalkar SR. Review on medicinal plants to target and inhibit the epidermal growth factor receptor signaling in cancer and tissue repair therapy. Int J Adv Pharm Biol chem. 2014; 3(1): 210-3.

2. Gupta A, Khamkar P, Chaphalkar S. Applications and uses of active ingredients from medicinal plants. Ind J Nov Drug Deli. 2014; 6: 106-11.

3. Gupta A, Chaphalkar SR. Immuno pharmacological activity of flavonoids isolated from Mesua ferreaFicus benghalensis and Jasminum auriculatum. Curr Life Sci. 2016; 2(2): 49-54.

4. Ramachandran B, Kamaraj M, Subramani V, Jeyakumar JJ. Screening of Phytochemistry and Secondary Metabolites: A Case Study on Nyctanthes arboritis. Int J Pharma Res Rev. 2014; 3(3): 7-11.

5. Kalam S, Gul MZ, Singh R and Ankati S. Free Radicals: Implications in Etiology of Chronic Diseases and Their Amelioration through Nutraceuticals. Pharmacol. 2015; 6(1): 11-20.

6. Rahman K. Studies on free radicals, antioxidants and co-factors. Clin Interv Aging. 2007; 2(2): 219-36.

7. Lai HC, Singh NP, Sasaki T. Development of artemisinin compounds for cancer treatment. Invest New Drugs. 2013; 31(1): 230-46.

8. Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT. Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc. 1971; 93(9): 2325-7.

9. Ayoola G, Coker H, Adesegun S, Adepoju-Bello A, Obaweya K, Ezennia E, 
et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop J Pharm Res. 2008; 7(3): 1019-24.

10. Gupta A. Terpenoids from three medicinal plants and their potential anti-inflammatory and immunosuppressive activity on human whole blood and peripheral blood mononuclear cells. Asian J Ethnopharmacol Med Foods. 2016; 4(1): 13-17.

11. Gupta A, Khamkar PR, Chaphalkar SR. In vitro anti-inflammatory activity of root aqueous extract of Mesua ferrea in human whole blood and peripheral blood mononuclear cells using flow cytometry. Int J pharmacy life Sci. 2014; 5(10): 3903-9.

12. Gupta A, Chaphalkar SR. Inhibitory potential of aqueous extract of Ficus bengalensis on human peripheral blood mononuclear cells. J PharmSci. 2014; 3(11): 250-3.

13. Gupta A, Chaphalkar SR. Anti-inflammatory and anti-microbial activities of aqueous leaves extract of Butea frondosa. J Herb Med Pharmacol. 2016; 5 (2): 7-11.

14. Soman I, Mengi SA, Kasture SB. Effect of leaves of Butea frondosa on stress, anxiety, and cognition in rats. Pharmacol Biochem Behav. 2004; 79(1): 11-6.

15. Gupta A, Chaphalkar SR. Anti-inflammatory activity of aqueous extract of leaves of Prosopis spicigera. Int J Res pharmacy life Sci. 2015; 3(1): 829-34.

16. Gupta A, Khajuria A, Singh J, Bedi KL, Satti NK, Dutt P, et al. Immunomodulatory activity of biopolymeric fraction RLJ-NE-205 from Picrorhiza kurroa. Int Immunopharmacol. 2006; 6(10): 1543-9.

17. Smirnoff N, Wheeler GL. Ascorbic acid in plants: Biosynthesis and function. Crit Rev Biochem Mol Biol. 2000; 35(4): 291-314.

18. Gupta A, Chaphalkar SR. Potential immunosuppressive and anti-inflammatory activity of aqueous extract of Mangifera indica. Adv Herb Med. 2015; 1(4): 47-54.

19. Gillett NA, Chan C. Applications of immunohistochemistry in the evaluation of immunosuppressive agents. Hum Exp Toxicol. 2000; 19(4): 251-4.