Antimicrobial studies of Ficus benghalensis and Ficus racemosa on pathogenic viral diseases

Document Type: Original Article

Authors

1 VSBT, Baramati

2 VSBT Baramati

Abstract

Background and aims: Medicinal plant products are considered to be an effective candidate against the number of viral diseases as generally observed or reported in developing countries. As per the literature, secondary metabolites (i.e. Alkaloids, flavonoids, saponins etc.) are reported in medicinal plant products and showed its antiviral properties. In this study, our group focused on those medicinal plants especially roots of Ficus benghalensis and Ficus racemosa related to New castle Disease Virus (NDV) and Infectious Bursal Disease (IBD) having in vitro antiviral activity. These studies were conducted on the human peripheral blood mononuclear cells (PBMC). 
Methods: For antimicrobial studies, different medicinal plant products especially roots of Ficus benghalensis and Ficus racemosa were collected from Vidya Pratishthan’s garden, School of Biotechnology, Baramati. These medicinal plant leaves are used in the form of aqueous extract and determined its anti-microbial activity against poultry viruses (i.e. NDV and IBD) in PBMC and determined proliferation assay, Th1 (TNF alpha) cytokine production and CD14 monocyte surface marker. 
Results: Three medicinal plant aqueous extracts showed noteworthy antimicrobial activity with respect to decline in proliferation assay, TNF alpha production and CD14 monocyte surface marker in human PBMC as compared to control. 
Conclusion: Some of the medicinal plant products have shown antimicrobial activity. Further immunobiological research is mandatory to elucidate the most active constituents that are present in these aqueous extracts which may be useful or target molecule in the development of new effective and safe antiviral agents.

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Main Subjects


1. Eldaghayes I, Rothwell L, Williams A, Withers D, Balu S, Davison F, et al. Infectious bursal disease virus: Strains that differ in virulence differentially modulate the innate immune response to infection in the chicken bursa. Viral Immunol. 2006; 19(1): 83-91.

2. Gupta A, Chaphalkar SR. Immunopharmacological activity of Zingiber officinal on human peripheral blood mononuclear cells. Asian J Med Pharmac Res. 2015; 5(2): 13-7.

3. Gupta A, Chaphalkar SR. Inhibitory potential of aqueous leaves extract of Mesua Ferrea and Mimusops Elengi on Antigen Specific Immune Response using Human Whole Blood. Asian J Med Pharm Res. 2015; 5(3): 22-6.

4. Liu Y, Wei Y, Wu X, Yu L. Preparation of ChIL-2 and IBDV VP2 fusion protein by baculovirus expression system. Cell Mol Immunol. 2005; 2(3): 231-5.

5. Fabian Z, Csatary CM, Szeberenyi J, Csatary LK. p53-independent endoplasmic reticulum stress-mediated cytotoxicity of a Newcastle disease virus strain in tumor cell lines. J Virol. 2007; 81(6): 2817-30.

6. Baumgart DC, Carding SR. Inflammatory bowel disease: Cause and immunobiology. Lancet. 2007; 369(9573): 1627-40.

7. Alviano DS, Alviano CS. Plant extracts: Search for new alternatives to treat microbial diseases. Curr Pharm Biotechnol. 2009; 10(1): 106-21.

8. Cohen ML. Epidemiology of drug resistance: Implications for a post-antimicrobial era. Science. 1992; 257(5073): 1050-5.

9. Gupta A, Chaphalkar S. Immunosuppressive terpenoids from extracts of Emblica officinalisFicus racemosa and Strychnos nux-vomica. Micromedicine. 2016; 2(1): 1-7.

10. Gupta A, Chaphalkar SR. Immunosuppressive activity of crude proteases from Ficus benghalensis on human whole blood using flow cytometry. As J Clinic Res. 2016; 2: 1-5.

11. Gupta A, Chaphalkar SR. Immunopharmacological activity of flavonoids isolated from Mesua ferrea, Ficus benghalensis and Jasminum auriculatum. Curr Life Sci. 2016; 2(2): 49-54.

12. Gupta A, Chaphalkar SR. Flow cytometric immunopharmacological studies of aqueous extract of Ficus racemosa as an immunomodulator. Sciences. 2016; 6(3): 108-14.

13. Bhandary SK, Kumari S, Bhat VS, Sharmila K, Bekal MP. Preliminary phytochemical screening of various extracts of Punica granatum peel, whole fruit and seeds. J Health Sci. 2012; 2(4): 35-8.

14. Gupta A, Khajuria A, Singh J, Bedi KL, Satti NK, Dutt P, et al. Immunomodulatory activity of biopolymeric fraction RLJ-NE-205 from Picrorhiza kurroa. Int Immunopharmacol. 2006; 6(10): 1543-9.