Antimicrobial activity of methanol extract of Citrullus colocynthis against antibiotic-resistant Staphylococcus aureus

Document Type: Original Article

Authors

1 Department of obstetrics and gynecology, maternal and fetal health research center, Zabol University of Medical Sciences, Zabol, Iran

2 2. Department of Biology, Faculty of Science, Payam Nor ,University of Tehran, Tehran, Iran

3 3. Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

4 Department of Biology- Faculty of Scienc- Shahid Bahonar University of Kerman- Kerman- Iran.

Abstract

Background and aims: Staphylococcus aureus and anaerobic gram-positive cocci, are medically the most important species in the genus Staphylococcus. Sometimes, the bacteria are called S. aureus. The constantly growing antibiotic-resistant bacterium is very important in treatments. The aim of this study is to evaluate the antimicrobial activity of ethanol extract from Citrullus colocynthis against the S. aureus resistant to antibiotics.
Methods: S. aureus strains were isolated from the city of Zabol and C.colocynthis extract was prepared using rotary devices and the MIC and MBC were determined by microdilution method.
Results: The results of this study showed that the extract from C. colocynthis inhibits the growth of bacteria in different concentrations, and that despite the relative strength of most species with different concentrations, the highest sensitivity was observed in concentrations of 10 and 20 mg/ml, in which is 100% bacteria were gone. C. colocynthis extract at a concentration of approximately 5 mg /ml had the highest inhibitory effect.
Conclusion:  The results showed high antimicrobial effects of ethanol extract of C. colocynthis, which can offer appropriate drug therapy.

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1. Fallah Hosseini DH, Kianbakht S, Mousavi R, Amnzadeh N. Investigating the Effect of Processing with Vinegar and Salty Water on Toxicity and Anti-Diabetic Effect of Hanzal Extract in Rats. Med Plants Quart.  2011; 4(40): 119-123.

2.Jafarnia S, Khosroshahi S,  Ghasemi M. Comprehensive Pictorial Guide to properties and uses of medicinal plants. Green Agri  ped Complex of Iran, 2006; 1-179.

3.Cecchini T. Encyclopedie des plantes medicinalis. Paris, 1979. 125-30 Neu HC. The crisis of antibiotic resistance. Scie. 1992; 257: 1061-9.

4. Ros JL, Recio MC. Medicinal plants and antimicrobial activity. J of Ethno. 2005; 100: 80–84.

5. Doss A, Vijayasanthi M, Anand SP, Parivuguna V, Venkataswamy R. Screening of Antimicrobial activity of essential oil and methanol extracts of Citrullus colocynthis (L.) Schrad. South As J Biol Sci. 2011; 1(1): 7- 15.

6.  Marzouka B,  Marzoukb Z, Décorc R, Edziri a H, et al. Antibacterial and anticandidal screening of Tunisian Citrullus colocynthis Schrad. from Medenine. J of Ethno. 2009; 125: 344–349.

7.  Gurudeeban S, Rajamanickam E, Ramanathan  T,  Satyavani. Kantimicrobial activity of  Citrullus colocynthis  in gulf of mannar. Inter J of Curr Res. 2011; 2: 078-081.

8. Shyamala growri S, Priyavardhini S, Vasantha K, Umadevi M. Antibacterial activity on Citrullus colocynthis leaf extracts. Anci  Sci of Life. 2009; 29(1): 12-13.

9. John Peter Paul J. Studies on Antimicrobial Efficiency of Citrullus colocynthis (L.) Schrad: A Medicinal Plant. Ethno Leaflets. 2008;  12: 944-47.

10. Mehni AM, Ketabchi S, Shahidi Bonjar GH. Antibacterial activity and polyphenolic content of Citrullus color. Internationa; j of Bosci. 2014; 4(3): 190-196.

11. L. Alkamel M. Antimicrobial activity of aqueous extract of Citrullus colocynthis L. fruit. Tikrit J of Phar Sci. 2005, 1(2):9- I 5.

12. Gowri SS, Priyavardhini S, Vasantha K, Umadevi M. Antibacterial activity on Citrullus colocynthis Leaf extract. Anc Sci Life. 2009;29(1):12-3.

13. Shawkey AM, Abdulall AK, Rabeh MA , Abdellatif AO. Enhanced biocidal activities of Citrullus colocynthis aqueous extracts by green nanotechnology. Inter J of Applied Res in Natural Produc. 2014; 7(2): 1-10

14. Najafi S, Sanadgol N, Sadeghi Nejad B, Ashofteh Beiragi M and Sanadgol E. Phytochemical screening and antibacterial activity of Citrullus colocynthis (Linn.) Schrad against Staphylococcus aureus. J of Med Plants Res. 2010; 4(22): 2321-2325,