Qualitative and quantitative changes of essential composition in the flowers of some populations of Elaeagnus angustifolia

Document Type: Original Article


1 Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

2 Department of Phytochemistry, Faculty of Chemistry, Azarbaijan Shahid Madani University, Tabriz, Iran


Background and aims: Elaeagnus angustifolia L. is a member of the Elaeagaceae family; different parts of it, especially fruits have been used for the treatment of several diseases in traditional medicine. The aim of this study was to isolate and determine essential oil composition of flowers of E. angustifolia collected from different ecological areas of East-Azarnayjan in Iran.
Methods: In this experimental study, the essential oils of the flower were isolated by hydrodistillation method and analyzed by GC and GC/MS.
Results: The number of compounds in the essential oil isolated from the population of Ahar, Marand and Hashtroud were 22, 17 and 14, respectively. The major component of all of the populations was ethylcinnamate; Ahar (47.59%), Marand (69.99%) and Hashtroud (85.49%). It was observed that the oil number of E. angustifolia decreases from 22 to 14 when the altitude increases from 1344-1750 m.
Conclusion:Chemical composition of the essential oils of E. angustifolia L. such as esters and aromatic acids contents were increased while the ketone content was decreased with increasing altitude.


Main Subjects

1. Klich MG. Leaf variations in Elaeagnus angustifolia related to environmental heterogeneity. Environ Exp Bot. 2000; 44(3): 171-83.

2. Hosseinzadeh H, Ramezani M, Namjo N. Muscle relaxant activity of Elaeagnus angustifolia L. fruit seeds in mice. J Ethnopharmacol. 2003; 84(2-3): 275-8.

3. Iriondo JM, De La Iglesia M, Perez C. Micropropagation of Elaeagnus angustifolia from mature trees. Tree Physiol. 1995; 15(10): 691-3.

4. Nikolaeva A, Krivenchuk P, Prokopenko A. Phenolic compounds of Elaeagnus angustifolia.J Khimiya Prirodnykh Soedineni. 2005; 6(6): 256.

5. Karimi G, Hosseinzadeh H, Rassoulzadeh M, Razavi BM, Taghiabadi E. Antinociceptive effect of Elaeagnus angustifolia fruits on sciatic nerve ligated mice. J Basic Med Sci. 2010; 13(3): 97-101.

6. Gurbuz I, Ustun O, Yesilada E, Sezik E, Kutsal O. Anti-ulcerogenic activity of some plants used as folk remedy in Turkey. J Ethnopharmacol. 2003; 88(1): 93-7.

7. Zargari A. Medicinal plants. Tehran: Tehran University Press; 1990; 4: 27-38.

8. Pearce CM, Smith DG. Plains cottonwood's last stand: Can it survive invasion of Russian olive onto the Milk River, Montana floodplain? Environ Manage. 2001; 28(5): 623-37.

9. Ramezani M, Hosseinzadeh H, Daneshmand N. Antinociceptive effect of Elaeagnus angustifolia fruit seeds in mice. J Ethnopharmacol. 2003; 84(13): 275-80.

10. Pirbalouti AG, Malekpoor F, Enteshari S, Yousefi M, Momtaz H, Hamedi B. Antibacterial activity of some folklore medicinal plants used by Bakhtiari tribal in Southwest Iran. Intl J Biol. 2010; 2(2): 55.

11. Jinshun L. Analysis of volatile and semi-volatile components from flower of Elaeagnus angustifolia. Sci Silvae Sin. 2007; 43(3): 122.

12. Hongjian Y, Huaiqin Z. Analysis of chemical constituents of the Elaeagnus angustifolia L. flowers by GC/MS. J Chinese Mass Spectrometry. 1989; 10(2): 59.

13. Goncharova NP, Glushekova AI. Extract of Elaeagnus angustifolia flowers. Chem Nat Compd. 2009; 33(3): 276-7.

14. Wang Y, Zhaokun ZP. Gas chromatographic-mas spectrometric analysis of aroma compositions from flowers of Elaeagnus angustifolia L. extracted by supercritical Co_2 fluid. J Flavour Fragrance Cosmetics; 2007; 3(1): 72.

15. Bekker N, Glushenkova A. Components of certain species of the Elaeagnaceae family. Chem Nat Compd. 2001; 37(2): 97-116.

16. Adams RP. Identification of essential oil components by GC/. J Am Soc Mass Spectrom. 2006; 16(11): 22-9.

17. Masada Y. Analysis of essential oils by gas chromatography and mass spectrometry. Chem Nat Compd. 2003; 32(2): 33-6.

18. Li HJ, Yang J. GC/MS analysis of chemical composition of volatile oil from flowers of Elaeagnus angustifolia. J Food Sci. 2001; 58(11): 275-7.

19. Wang CQ. Chemical composition of the flower essential oils Elaeagnus angustifolia. Adv Mat Res. 2012; 34(6): 162-4.

20. Chen Y, Guo-qiang G. Gas chromatographic-mass spectrometric analysis of essential oil extracted by supercritical CO2 from flowers of Elaeagnus angustifolia L. Food Sci. 2013; 34(5): 152-6.

21. Junhua H, Chaofeng W. Present situation and prospect about the study of Elaeagnus angustifolia L. J Chinese Wild Plant Res. 2005; 3(2): 663-5.

22. Li Z, Ning C, Dunyuan  X, Haiquan  L, Yaozu C. Study on chemical constituents of volatile oil of fresh flowers of Elaeagnus Angustifolia L. Chem Res Chin Univ. 1989; 10(8): 804-8.

23. Bucur L, Stanciu G, Istudor V. The
GC-MS analysis of Elaeagnus angustfolia L. flowers essential oil. Chem Nat Compd. 2007; 58(11): 706-9.