The measurement of the quercetin of different parts of Tribulus terrestris by HPLC

Document Type: Original Article


1 1Agronomy Dept., Ramin University of Agriculture and Natural Resources, Ahwaz, I.R. Iran

2 2Student, Ramin University of Agriculture and Natural Resources, Ahwaz, I.R. Iran

3 3Student, Psychosocial Injuries Research Center, Ilam University of Medical Sciences, Ilam, I.R. Iran

4 4Psychosocial Injuries Research Center, Ilam University of Medical Sciences, Ilam, I.R. Iran


Background and aims: Tribulus terrestris fruit, leaf, and root have medical effects in the treatment of cancer, viral infections and prevention of cardiovascular diseases. The present study was aimed to evaluate the quercetin flavonoid levels from different parts of the Tribulus terrestris collected from different regions of Khuzestan in 2014. Methods: In this experimental study, four parts of the Tribulus terrestris including; fruits, leaves, stems and roots were collected from different regions of Khuzestan including Shushtar, Mollasani and Andimeshk. The analysis was carried out to compare the chemical profile of the different extracts of Tribulus terrorists using reverse phase HPLC with UV detector. The mobile phase that consisted of phosphoric acid buffer with pH=3 and acetonitrile was used for isocratic elution. The flow rate was adjusted to 1.0 ml/min. The detection wavelength was at 203 nm. All separations were performed at ambient temperature. Results: The results reported that the quercetin flavonoid level were highest in the Andimeshk leaves samples (69.57427 ppm). However, the Andimeshk fruits samples (4.141953 ppm) have the lowest levels of the quercetin flavonoid. Conclusion: Considering the cost effectiveness in extracting compounds from medicinal plants, it is recommended to identify the highest level of the quercetin flavonoid in each region and in each part of the plant.


Main Subjects


1. Mozaffarian V. Flora of Khuzestan. Tehran: Research Center of Natural Resource and Husbandry of Khuzestan Pub; 1999.

2. Abirami P, Rajendran A. GC-MS Analysis of Tribulus terrestris. Asian J Plant Sci Res. 2011; 1(4): 13-6.

3. Mozaffarian V. Trees and shrubs of Iran. Tehran: Farhang Moaser Pub; 2005.

4. Trease G, Evans W. Trease and Evans Pharmacognosy. A taxonomic approach to the study of medicinal plants and animal derived drugs. 15th ed. Singapore: Harcourt Brace and Company Asia Pvt Ltd; 2002.

5. Antonio J, Uelmen J, Rodriguez R, Earnest C. The effects of Tribulus terrestris on body composition and exercise performance in resistance-trained males. Int J Sport Nutr Exerc Metab. 2000; 10(2):208-15.

6. Rogerson S, Riches CJ, Jennings C, Weatherby RP, Meir RA, Marshall-Gradisnik SM. The effect of five weeks of Tribulus terrestris supplementation on muscle strength and body composition during preseason training in elite rugby league players. J Strength Cond Res. 2007; 21(2): 348-53.

7. Abirami P, Rajendran A. GC-MS analysis of Tribulus terrestris. l. Asian J Plant Sci Res. 2011; 1(4): 13-6.

8. Usman H, Musa YM, Ahmadu AA, Tijjani MA. Phytochemical and antimicrobial effects of Chrozophora senegalensis. Afr J Tradit Complement Altern Med. 2007; 4(4): 488-94.

9. Kostova I, Dinchev D. Saponins in Tribulus terrestris-chemistry and bioactivity. Phytochem Rev. 2005; 4(2): 111-37.

10. Wu T, Shi L, Kuo S. Alkaloids and other constituents from Tribulus terrestris. Phytochemistry. 1999; 50(8): 1411-5.

11. Louveaux A, Jay M, Taleb O, Hadi M, Roux G. Variability in flavonoid compounds of four Tribulus species: Does it play a role in their identification by desert locust Schistocerca gregaria? J Chem Ecol. 1998; 24(9): 1465-81.

12. Matin Y, Alavi S, Hajiaghaee R, Ajani Y. Flavonoid Glycosides from Tribulus terriestris L. orientalis Iran. J Pharm Sci. 2008; 4: 231-6.

13. Middleton E, Kandaswami C. The impact of plant flavonoids on mammalian biology implications for immunity, inflammation and cancer. In: Harborne JB. The flavonoids. London: Chapman and Hall; 1993.

14. Jaimand K, Rezaee M, Asareh M, Tabaei Aghdaei S, Meshkizadeh S. Extraction and determination of Kaempferol and Quercetin in petals of 10 genotypes of Rosa damascena Mill, from western Iran. Iran J Medicinal Aromatic Plants. 2010; 25(4): 547-55.

15. Ivanova A, Lazarova I, Mechkarova P, Semerdjieva I, Evstatieva L. Intraspecific variability of biologically active compounds of different populations of Tribulus terrestris in Thracian floristic region. Biotechnol Biotechnol Equip. 2011; 25(2): 2357-61.

16. Srivastava AW, Shym S. Citrus: Climate and soil: International Book Distributing Company; 2002.

17. Boots AW, Haenen GR, Bast A. Health effects of quercetin: From antioxidant to nutraceutical. Eur J Pharmacol. 2008; 585

(2-3): 325-37.

18. Harwood M, Danielewska-Nikiel B, Borzelleca JF, Flamm GW, Williams GM, Lines TC. A critical review of the data related to the safety of quercetin and lack of evidence of in vivo toxicity, including lack of genotoxic/carcinogenic properties. Food Chem Toxicol. 2007; 45(11): 2179-205.

19. Chuang CC, Martinez K, Xie G, Kennedy A, Bumrungpert A, Overman A,
et al. Quercetin is equally or more effective than resveratrol in attenuating tumor necrosis factor-{alpha}-mediated inflammation and insulin resistance in primary human adipocytes. Am J Clin Nutr. 2010; 92(6): 1511-21.

20. Sakharkar BM. Principles of hospital administration and planning. New Delhi. India: Jaypee Brothers Medical Pub; 2009.