Antibacterial activity of Marrubium vulgare L. against antibiotic resistance Klebsiella pneumoniae strains

Document Type: Original Article

Authors

1 1Gynecology Dept., Zabol University of Medical Sciences, Zabol, I.R. Iran

2 2Medical Molecular Genetics Dept., Shahid Sadoughi University of Medical Sciences, Yazd, I.R. Iran

3 3Laboratory Expert Institute of Plant Biotechnology, University of Zabol, Zabol, I.R. Iran

4 4Plant Protection Dept., and Institute of Plant Biotechnology, University of Zabol, Zabol, I.R. Iran.

Abstract

  Background and aims: Herbal medicines are the major remedy in traditional medical systems and have a great contribution in maintaining human health and preventing many infectious diseases. The aim of this study was to assay antibacterial potential of Marrubium vulgare L. extract against Klebsiella pneumoniae resistant strains to current antibiotics and also GC/MS analysis to better understanding the extract composition. Methods: In this experimental research, 30 K. pneumoniae strains isolated from urine culture of hospitalized patients were used. The essential oil of Marrubium vulgare L. was obtained by hydro distillation for 2 hours using the Celevenger with yield of 75%. Methanolic extract from M. vulgare L. was prepared using Rotary apparatus. In order to determine chemical composition of essential oil, gas chromatography coupled with mass spectrometry (GC-MS) was performed. The minimum inhibitory concentrations and minimum bacterial concentrations were investigated to characterize the antimicrobial activities of this essential oil and its extract. Data were analyzed using analysis of variance (one-way) to determine the statistical differences between different tests. Results: The results showed that K. pneumoniae strains were resistant to 4 or 3 agents including: Ampicillin (65%), Gentamicin (30%), Sulfamethoxazol (25%). The lowest and the highest MIC value of M. vulgare L. extract were 2.5 and 10 mg/mL, respectively. The highest and the lowest MIC value of M. vulgare L. essential oil was 5 and 1/25 mg/m respectively. Conclusion: The present study confirmed that essential oil and extract of this plant could be served as an antibacterial agent in pharmaceutical industry.  

Keywords

Main Subjects


1. Youn YS, Lee KY, Hwang JY, Hwang JY, Rhim JW, Kang JH, Lee JS. Difference of clinical features in childhood Mycoplasma pneumoniae pneumonia. BMC Pediatr. 2010; 10: 48-55.

2. Zarai Z, Kadri A, Ben Chobba I, Ben Mansour R, Bekir A, Mejdoub H, et al. The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia. Lip Health Dis. 2011; 10: 161-65.

3. Meyre-Silva C, Cechinel-Filho V. A review of the chemical and pharmacological aspects of the genus marrubium. Curr Pharm Des. 2010; 16(31): 3503-18.

4. Morteza-Semnani K, Saeedi M, Babanezhad E. The Essential Oil Composition of Marrubium vulgare L. from Iran. J Essential Oil Res. 2008; 20(6): 488-90.

5. Bauer AW, Kirby WM, Sherris JC, Turck, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(3): 493-56.

6. Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc. 2008; 3(2): 163-75.

7. Kadri A, Zarai Z, Bekir A, Gharsallah N, Damak M, Gdoura R. Chemical composition and antioxidant activity of Marrubium vulgare L. essential oil from Tunisia. Afr J Biotech. 2011; 10(19): 3908-14.

8. Zawiślak G. Chemical composition of essential oils of Marrubium vulgare L. and Marrubium incanum Desr. grown in Poland. Chemija. 2012; 23(2): 136-140.

10. Masoodi MH, Ahmed B, Zargar IM, Khan SA, Khan S, Singh P. Antibacterial activity of whole plant extract of Marrubium vulgare. Afr J Biotech. 2008; 7(2): 86-87.

11. Buzayan MM, Egarbulleir F. Antibacterial activity of medicinal aqueous plant extracts against Mycobacterium tuberculosis. Malays J Microbiol. 2012; 8: 203-06.

12. Kanyonga PM, Faouzi MA, Meddah B, Mpona M, Essassi EM, Cherrah Y. Assessment of methanolic extract of Marrubium vulgare for anti-inflammatory, analgesic and anti-microbiologic activities. J Chem Pharm Res. 2011; 3: 199-204.

13. Daferera DJ, Ziogas BN, Polissiou MG. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis. Crop Protect. 2003; 22(1): 39-44.