Phytochemical composition and antibacterial properties of the essential oil of Achillea biebersteinii Afan. (Asteraceae)

Document Type: Original Article


1 Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Biology, University of Tabriz, Tabriz, Iran


aims: The aim of this study was to characterize the chemical composition and antimicrobial properties of Achillea biebersteinii essential oil (EO).
Methods: The chemical composition of samples obtained from Marand city in East Azerbaijan, was assessed using gas chromatography-mass spectrometry (GC-MS). The antimicrobial properties were evaluated by the disc diffusion method against methicillin-resistant Staphylococcus aureus (MRSA), other extended-spectrum beta-lactamases (ESBLs) producing, as well as Gram-negative and Gram-positive bacteria. The minimum inhibitory concentration (MIC) value of EO was assessed using the agar dilution method.
Results: In A. biebersteinii the major compounds were α-terpinene (41.42%), 2-carene (13.96%), m-cymene (13.41%) and 1,8-cineole(8.91%).The EO showed antimicrobial activity against ten microorganisms, especially Streptococcus sanguis, S. aureus (MRSA strain), and Klebsiella pneumoniae (ESBL-producing strain), which was potentially better than tetracycline and kanamycin.
Conclusion: This study confirmed that EO of A. biebersteinii has in vitro antimicrobial activity against Gram-negative and Gram-positive bacteria, which has made it an alternative antibacterial agent.


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